HPLC法检测抗氧化包装膜中槲皮素的保留率

Determination of Quercetin Retention in Antioxidant Food Package Film by High Performance Liquid Chromatography

中文关键词: 槲皮素  高效液相色谱  食品包装膜  抽提  含量测定

英文关键词: quercetin  high performance liquid chromatography（HPLC）  food package film  extraction  content determination

中文摘要:

      目的 建立一种快速准确地测定在含有槲皮素的食品抗氧化包装膜中槲皮素保留率的测定方法。 方法 在温度为 65 ℃的纯乙醇溶液中， 将含有槲皮素的食品薄膜抽提10 h， 重复 1 次抽提过程，得到含槲皮素的抽提液， 采用高效液相色谱法测定抽提液中槲皮素的含量。 结果 槲皮素回归方程为y=643.65 x+0.0572（ R2=0.9995， n=6）， 在槲皮素的质量浓度为 1.6~280 μg/mL时线性良好， 平均加样回收率为 99.69%（RSD=1.77%）。 结论 该检测方法出峰快， 能有效改善峰形， 避免拖尾峰的出现。 该方法简便快速、易操作、准确可靠，可用于检测抗氧化食品包装膜中槲皮素的保留率。

英文摘要:

      Objective To establish a method for the determination of quercetin retention in antioxidant food package film by HPLC. Methods Quercetin was extracted in ethanol at 65 ℃ for 10 h and the extraction was repeated once. Results The detection method was fast, and could effectively improve the peak shape as well as avoid peak tailing. The linear regression equation for quercetin was y=643.65x+0.0572 (R2=0.9995, n=6) and the linear range was 1.6~280 μ g/mL. The average recovery was 99.69% (RSD=1.77%) for quercetin. Conclusion This was a simple, rapid and accurate method to detect the quercetin retention in the antioxidant food package film.