葛文亮,林璐,胥传来.双酚A二环氧甘油醚的免疫检测方法研究[J].包装工程,2019,40(23):59-63. GE Wen-liang,LIN Lu,XU Chuan-lai.Immunoassay Method of 2,2-bis (4-glycidyloxyphenyl) Propane[J].Packaging Engineering,2019,40(23):59-63. |
双酚A二环氧甘油醚的免疫检测方法研究 |
Immunoassay Method of 2,2-bis (4-glycidyloxyphenyl) Propane |
投稿时间:2019-09-26 修订日期:2019-12-10 |
DOI:10.19554/j.cnki.1001-3563.2019.23.009 |
中文关键词: 双酚A二环氧甘油醚 单克隆抗体 ic-ELISA 加标回收 |
英文关键词:2,2-bis (4-glycidyloxyphenyl) propane monoclonal antibody ic-ELISA standardized recovery |
基金项目:国家重点研发计划(2018YFF0214803-04) |
作者 | 单位 |
葛文亮 | 1.无锡市第二人民医院,江苏 无锡 214002 |
林璐 | 2.江南大学食品学院,江苏 无锡 214122 |
胥传来 | 2.江南大学食品学院,江苏 无锡 214122 |
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Author | Institution |
GE Wen-liang | 1.Wuxi No.2 People's Hospital, Wuxi 214002, China |
LIN Lu | 2.School of Food Science and Technology, Jiangnan University, Wuxi 214122, China |
XU Chuan-lai | 2.School of Food Science and Technology, Jiangnan University, Wuxi 214122, China |
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中文摘要: |
目的 快速检测湖水中的双酚A二环氧甘油醚。方法 试验设计一种新型双酚A二环氧甘油醚半抗原,并成功与载体蛋白偶联,经过小鼠免疫、细胞融合技术和三次亚克隆等方法,成功筛选出了可以稳定分泌BADGE单克隆抗体的杂交瘤细胞株。结果 成功制备了高灵敏度高特异性的双酚A二环氧甘油醚单克隆抗体,可以用于针对双酚A二环氧甘油醚的间接竞争酶联免疫吸附测定(ic-ELISA)。基于单克隆抗体,绘制了标准曲线,结果显示,单克隆抗体的IC50=1.15 ng/mL,检测线性范围IC20~IC80为0.16~8.15 ng/mL。通过加标回收实验,BADGE在湖水的添加回收率均在80%~120%之间。结论 双酚A二环氧甘油醚单克隆抗体在实际湖水样品检测中的运用具有可行性。 |
英文摘要: |
The objective is to rapidly detect 2,2-bis (4-glycidyloxyphenyl) propane in lake water. A novel 2,2-bis (4-glycidyloxyphenyl) propane hapten was designed and successfully coupled with carrier protein. Hybridoma cell lines capable of stably secreting BADGE monoclonal antibody were successfully screened by the methods of mouse immuniza-tion, cell fusion technology and three subclones. A high sensitivity and specificity monoclonal antibody against 2,2-bis (4-glycidyloxyphenyl) propane, which can be used for indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) of 2,2-bis (4-glycidyloxyphenyl) propane was prepared successfully. Based on monoclonal antibody, the standard curve was drawn. The results showed that the IC50 of monoclonal antibody was 1.15 ng/mL, and the linear range of IC20-IC80 was 0.16-8.15 ng/mL. The recovery rate of BADGE in lakewater was between 80% and 120%. In conclusion, the application of 2,2-bis (4-glycidyloxyphenyl) propane monoclonal antibody in the detection of lake water is feasible. |
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