SERS技术在食品有毒物质检测中的应用

Application of SERS Technology in Detection of Food Toxicant

中文关键词: 辛硫磷  毒死蜱  双酚A  表面增强拉曼光谱  金纳米材料

英文关键词: phoxim  chlorpyrifos  bisphenol A  surface-enhanced Raman spectroscopy  gold nanoparticles

中文摘要:

      目的 开发一种新型检测手段，实现对果蔬等食品中有毒物质的高灵敏度检测。方法 以高“SERS”活性的多枝状纳米金为SERS基底，将负载辛硫磷、毒死蜱和双酚A的底物进行拉曼检测，收集不同检测物的标准图谱并进行定性分析。分别构建不同物质的定量检测模型。将上述方法引入实际食品样品的有毒物质定性检测中进行实用性实验。结果 实验成功收集到可用于定性检测的3种物质的SERS光谱，使用该方法检测到上述物质的检出限分别为4.97，0.565，0.594 ng/L。将上述方法应用于实际样品检测中，如果蔬中辛硫磷和毒死蜱的检测，以及塑料包装饮品中双酚A残留量的检测，测得的回收率均超过80%。结论 研究结果为降低果蔬等制品中辛硫磷、毒死蜱和双酚A的检出限提供了理论依据。

英文摘要:

      The work aims to explore a novel detection approach to realize the high-sensitivity detection of toxicants in foods like fruit and vegetable. The multi-branched nano-gold with high SERS activity was taken as SERS substrate, and substrates loaded with phoxim, chlorpyrifos and bisphenol A were subjected to Raman detection to collect the standard spectra of different detection objects for qualitative analysis. Quantitative detection models of different substances were constructed. The above approach was introduced to the toxicant qualitative detection of actual food samples for practical experiment. The DERS spectra of three substances for qualitative detection were successfully collected through experiment and the detection limit of above substances by the approach was 4.97, 0.565 and 0.594 ng/L respectively. When the above approach was used in the detection of actual sample, the recovery rate of phoxim and chlorpyrifos in fruit and vegetable and the residues of bisphenol A in drinks with plastic package was over 80%. The research result provides a theoretical basis for reducing the detection limit of phoxim, chlorpyrifos and bisphenol A in foods like fruit and vegetable.