精氨酸对引起采后蓝莓腐烂的链格孢靶标菌的抑制效果研究

Effect of L-arginine on the Alternaria Targets Causing Postharvest Blueberry Rot

中文关键词: 蓝莓  链格孢种群  致病性  L-精氨酸  抑菌效果

英文关键词: blueberry  Alternaria species  pathogenicity  L-arginine  inhibition effect

中文摘要:

      目的 旨在筛选引起蓝莓采后腐烂的链格孢靶标菌株，并比较L-精氨酸和山梨酸钾对其抑制效果，为蓝莓采后链格孢腐烂病的防控提供前期基础。方法 通过组织分离法结合单孢分离法进行致腐菌的分离，采用ITS序列分析进行属水平鉴定，采用ATP序列分析结合形态学观察进行种水平鉴定，采用平板抑菌法比较L-精氨酸和山梨酸钾的抑菌效果。结果 本研究分离获得的链格孢菌均有致病性，分别属于3个不同种群，分别为细极链格孢(Alternaria tenuissima)、互隔交链孢(A. alternata)和云南铁杉链格孢(A. dumosa)。以菌株B20190623E3(A. tenuissima代表菌株)、菌株B20190623B1(A. alternata代表菌株)、菌株B20190623C1(A. dumosa代表菌株)为靶标菌，发现L-精氨酸对供试3株靶标菌的EC50值均低于山梨酸钾。结论 供试链格菌株均有致病性，其中引起蓝莓采后腐烂的优势链格孢种群为细极链格孢。云南铁杉链格孢为引起蓝莓果实腐烂的新纪录种。筛选获得了3株链格孢代表靶标菌。L-精氨酸对引起蓝莓采后腐烂的链格孢菌具有较好的抑制效果。

英文摘要:

      The work aims to screen out the Alternaria targets causing postharvest blueberries rot and compare the inhibition effects of L-arginine and potassium sorbate on those Alternaria targets, so as to provide a preliminary basis for the prevention and control of postharvest blueberries rot caused by Alternaria targets. Tissue separation and single spore separation were used to isolate the Alternaria targets. ITS and ATP sequence analysis were adopted for the genus level identification and the species level identification, respectively. Plate inhibition method was used to compare the inhibition effects of L-arginine and potassium sorbate. All Alternaria isolates had pathogenicity and were distinguished to 3 Alternaria species, including A. tenuissima, A. alternata and A. dumosa. B20190623E3, B20190623B1 and B20190623C1 were selected out as targets for different Alternaria species. L-arginine had better inhibition effects than potassium sorbate on Alternaria targets. All the tested Alternaria targets are pathogenic, and A. tenuissima is the dominant causing postharvest rot of blueberry. A. dumosa is a new record species causing blueberry rot. Three representative targets of Alternaria are screened. L-arginine has a good inhibition effect on Alternaria causing postharvest rot of blueberry.